β-Galactosidase Assay (CPRG)
Click Image for Gallery
β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic o-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression.
β-galactosidase catalyzes the hydrolysis of the galactoside analog chlorophenol red-β-D-galactopyranoside (CPRG). Cell lysates are incubated with the supplied reagents and the β-Galactosidase converts the yellow-orange CPRG substrate into the red chromophore chlorophenol red, yielding a dark red solution. The β-galactosidase gene functions well as a reporter gene because the protein product is extremely stable, resistant to proteolytic degradation in cellular lysates, and easily assayed. The levels of activity β-galactosidase expression can be quickly measured by this method.
β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570–595 nm. Although the CPRG β-Galactosidase Assay Kit provides all the required reagents for 500 micro-assays in a 96-well plate, it is easily adapted for different assay size.
- Colorimetric Assay
- ~10X More Sensitive than ONPG Assays
- 500 Microwell Assays
- Monitor levels of transfection in cells
|Material Safety Data Sheet|
- Arikawa, H. and Matsumoto, K. (2016) Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator. Microb. Cell Fact. DOI: 10.1186/s12934-016-0583-7
- Wang, Jing-Hung et al (2014) Antimicrog Agents Chemother. doi: 10.1128/AAC.03683-14
|β-Galactosidase Assay (CPRG)||Kit||