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Glycoprotein Staining Kit

Catalog Description Size Price(USD) Qty
786-254
Glycoprotein Staining Kit 10 Mini Gels
$248.00

Glycoprotein Staining Kit

For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).

 

The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars.  The supplied oxidizing agent first oxidizes the cis-diol sugar groups to aldehydes.  The aldehyde groups react with the sensitive Glyco-Stain Solution forming Schiff bonds and producing strong magenta color bands. 

 

In addition to glycoprotein staining, the kit is supplied with RAPIDstain™, an enhanced Coomassie stain.  RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining.

 

The Glycoprotein Staining kit is highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included. In addition, the kit allows for the detection of glycosylated and non-glycosylated proteins on a single gel or membrane.

 

The Glycoprotein Staining kit is supplied with an enhance Coomassie stain, RAPIDstain™, that enhances the glycoprotein staining as well as staining non-glycosylated proteins (Figure 1B). 

 

The kit is sufficient for 10 mini gels (8 x 8cm) or 20 nitrocellulose membrane (8 x 8cm). 

 

 

Features

  • Specific glycosylated protein staining with optional non-glycosylated protein staining
  • Two stains in one kit
  • Nanogram level sensitivity
  • Stable periodate/Schiff staining

 


Applications

  • Improved detection of glycoproteins
  • Stain polyacrylamide gels or nitrocellulose membranes

 

 

Protocol
786-254
Material Safety Data Sheet
786-254
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Technical Literature
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
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