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OmniTemplate™

Catalog Description Size Price(USD) Qty
786-013
OmniTemplate™ 100 Preps
$170.00

OmniTemplate™

OmniTemplate™ is specifically designed for the rapid isolation of a DNA template for polymerase chain reaction (PCR) analysis from mammalian tissue samples, blood and cell cultures. This single tube method produces a high concentration, ready-to-use supply of DNA template for microanalysis, genotyping and a multitude of other applications.

The method involves isolation of nuclei and their subsequent digestion with LongLife™ Proteinase K in the same tube the samples were collected in. The isolated nuclei are digested to release template DNA in a buffer compatible with PCR and other downstream applications. The OmniTemplate™ samples can be directly used in PCR and other applications without further manipulations. One prep is approximately 1-10mg tissue.

Figure: OmniTemplate™ produces high quality genomic DNA template. 5μl (lanes 1, 3, 5) or 15µl (lanes 2, 4, 6) PCR reactions were loaded onto a 2% agarose gel. Template was purified from 104 (lanes 1-2), 106 (lanes 3-4) or 2.8x106 (lanes 5-6) NIH3T3 cells.

Features

 

 

 

 

  • Single tube genomic DNA isolation.
  • Compatible with PCR and other downstream applications.
  • Suitable for blood, cells, and animal tissue samples.
  • No toxic chemicals, no phenol, no hazardous waste.


Applications

 

 

 

 

  • Designed for the rapid preparation of DNA template for large throughput microanalysis.
  • High recovery of ready-to-use DNA templates in a short period of time.

 

Protocol
786-013
Material Safety Data Sheet
786-013
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Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.
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