The XIT™ Genomic DNA from Buccal Cells kit is designed for the isolation of genomic DNA from Buccal cells (cheek cells).
The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
The kit is available with (Cat. # 786-343, 786-344) or without (Cat. # 786-341, 786-342) cheek cell collection brushes. The kits without the brushes uses a mouthwash method to collect Buccal cells.
The kits are available in two different sizes. The purified DNA has a A260/A280 ratio between 1.8 and 2.0 and is up to 200kb in size. The yield is approximately 3-5µg/swab.
Isolate high quality genomic DNA
Protocols for brush or mouthwash cheek cell collection
Yield is approximately 3-5µg/swab
Suitable for archive quality DNA.