The XIT™ Genomic DNA from Yeast kit is designed for the isolation of genomic DNA from overnight yeast cultures.
The kit uses three main steps (see figure) that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
Two sizes of kit are available for processing a total of 25 and 250ml of bacterial culture. XIT™ Genomic DNA from Yeast Kit protocol is designed to use 1ml overnight culture, however the protocol can be easily adapted for larger tissue sample sizes. The purified DNA has a A260/A280 ratio between 1.7 and 1.9 and is up to 200kb in size. The yield is approximately 1-6µg/ml culture.
Isolate high quality genomic DNA
Suitable for Yeast cultures
Yield 1-6µg/mg tissue
Suitable for archive quality DNA.