Glycoprotein Staining Kit (6 Citations)
For the highly sensitive detection of glycoproteins following gel electrophoresis (Figure 1) or protein transfer to nitrocellulose membranes (Figure 2).
The kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent first oxidizes the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive Glyco-Stain Solution forming Schiff bonds and producing strong magenta color bands.
In addition to glycoprotein staining, the kit is supplied with RAPIDstain™, an enhanced Coomassie stain. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining.
The Glycoprotein Staining kit is highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included. In addition, the kit allows for the detection of glycosylated and non-glycosylated proteins on a single gel or membrane.
The Glycoprotein Staining kit is supplied with an enhance Coomassie stain, RAPIDstain™, that enhances the glycoprotein staining as well as staining non-glycosylated proteins (Figure 1B).
The kit is sufficient for 10 mini gels (8 x 8cm) or 20 nitrocellulose membrane (8 x 8cm).
- Specific glycosylated protein staining with optional non-glycosylated protein staining
- Two stains in one kit
- Nanogram level sensitivity
- Stable periodate/Schiff staining
- Improved detection of glycoproteins
- Stain polyacrylamide gels or nitrocellulose membranes
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|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|