HOOK™-NHS-LC-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.
For our complete HOOK™-NHS-LC-Biotin Labeling Kit, click here.
- Molecular Weight: 454.54
- Spacer Arm (Å): 22.4
- Reactive Group: NHS-ester
- Membrane Permeable : Yes
- Water Soluble: No
- Cleavable/ Reversible: No
- Reaction pH: 7-9
- For the coupling of biotin to protein amine groups
- For the labeling of proteins with NHS-LC-Biotin
|Material Safety Data Sheet|
|Assay Development||A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.|
|NHS Ester Reactivity Assay|
|Protein Labeling & Conjugation|