HOOK™-NHS-LC-LC-Biotin

HOOK™-NHS-LC-LC-Biotin

HOOK™ NHS-LC-LC-Biotin reacts with protein primary amines.  Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35 are available on the surface of the molecules and can be reacted with amine reactive esters.

 
For our complete HOOK™ NHS-LC-LC-Biotin Labeling Kit, click here.

 


Features

 Molecular Weight

 567.70

 Spacer Arm (Å)

 30.5

 Reactive Group

 NHS-ester

 Membrane Permeable

 Yes

 Water Soluble

 No

 Cleavable/ Reversible

 No

 Reaction pH

 7-9

 


Applications

  • For the labeling of proteins with NHS-LC-LC-Biotin
  • For the coupling of biotin to protein amine groups

 

 

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Protocol
BG-03
Material Safety Data Sheet
BG-03
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Technical Literature
Antibody Handbook
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
NHS Ester Reactivity Assay
Protein Labeling & Conjugation


Catalog Description Size Price(USD) Qty
BG-03
HOOK™-NHS-LC-LC-Biotin 50mg
$180.00