Heterobifunctional Cross-Linkers
Cross-linkers with two different reactive ends
ρ-Azidobenzoyl Hydrazide
Features
Molecular Weight: 177.19
Spacer Arm (Å): 11.9
Reactive Toward: Carbohydrates
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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N-5-Azido-2-nitrobenzyloxysuccinimide Features Molecular Weight: 305.20 Spacer Arm (Å): 7.7 Reactive Toward: Primary Amines Membrane Permeable: YES Water Soluble: NO Cleavable/ Reversible: No..
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APDP
A sulfhydryl-reactive and photoreactive cross-linker
Features
Chemical Name: N-[4-(p-Azidosalicylamido)butyl]-3’-(2’-pyridyldithio)propionamide
Synonym: APDP
Formula: C₁₈H₂₀N₆O₂
Molecular Weight: 446.55
Spacer Arm: 21.0Å
Reactive Toward: sulfhydryl and amino..
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Gene/Protein:
EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and i..
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N-(ε-Maleimidocaproic acid) hydrazide
Features
Molecular Weight: 225.24
Spacer Arm (Å): 11.8
Reactive Toward: Sulfhydryl + Carbohydrate
Membrane Permeable: Not tested
Water Soluble: NO
Cleavable/ Reversible: NO
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N-(ε-Maleimidocaproyloxy) succinimide ester
Features
Molecular Weight: 308.29
Spacer Arm (Å): 9.4
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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N-γ-Maleimidobutyryloxysuccinimide ester
Features
Molecular Weight: 280.23
Spacer Arm (Å): 6.8
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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Maleimide PEG succinimidyl carboxymethyl
Features
Molecular Weight: ~3400
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: Not tested
Water Soluble: NO
Cleavable/ Reversible: NO
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m-Maleimidobenzoyl-N-hydroxysuccinimide ester
Ideal for hapten-carrier protein, toxin-antibody, enzyme-antibody crosslinking
Features
Molecular Weight: 314.25
Spacer Arm (Å): 9.9
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
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N-Hydroxysuccinimidyl-4-azidosalicyclic acid
Features
Molecular Weight: 276.21
Spacer Arm (Å): 8.0
Reactive Toward: Primary Amines
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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N-(ρ-Maleimidophenyl) isocyanate
Features
Molecular Weight: 214.18
Spacer Arm (Å): 8.7
Reactive Toward: Sulfhydryl + Hydroxyl
Membrane Permeable: Not tested
Water Soluble: NO
Cleavable/ Reversible: NO
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N-Succinimidyl(4-iodoacetyl)aminobenzoate
Ideal for enzyme-antibody crosslinking
Features
Molecular Weight: 402.14
Spacer Arm (Å): 10.6
Reactive Toward: Primary Amine + Sulfhydryl
Membrane Permeable: YES
Water Soluble: NO
Cleavable/ Reversible: NO
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