Histidine-Tagged Protein Purification
G-Biosciences provide a range of Immobilized Metal Affinity Chromatography (IMAC) resins for 6X His tagged protein purification.
Four IMAC purification resins are offered:
Nickel Chelating Resin: The most commonly used IMAC purification resin for the purification of 6X His recombinant proteins that offers high binding efficiency and low non-specific binding.
Cobalt Chelating Resin: Growing increasingly popular due to its advantage over Nickel Chelating Resin. Although 6X His recombinant proteins bind with a slightly lower efficiency compared to Nickel Chelating Resin there is a significant reduction in non-specific binding. Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities.
Nickel and Cobalt chelating resins are available as a resin slurry or 0.2mL, 1mL, and 3mL spin columns.
Zinc Chelating Resin: For the purification on zinc binding proteins, including 6x His recombinant proteins. Available as a resin.
Copper Chelating Resin: For the purification on copper binding proteins, including 6x His recombinant proteins. Available as a resin.
Cobalt has the highest selectivity of the resins followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc.