Hot Start Taq DNA Polymerase
G-biosciences hot start Taq DNA Polymerase is chemically modified that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity. Hot start Taq DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity.
- E. coli strain expressing a Taq DNA Polymerase gene from Thermus aquaticus.
- Hot start Taq DNA Polymerase
- 10X PCR Buffer with Mg2+
- 5X GC Enhancer
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72º C.
10X PCR buffer with Mg2+:
100 mM Tris-HCl pH 9.0, 15 mM MgCl2, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630, 0.5% Tween 20, 50% Glycerol
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC