Human Multiple Tissue Blot I
The premade blots are prepared with the appropriate GenLysate and 50μg protein is loaded onto a 4-20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer.
The blots are ready to be blocked and probed with the antibodies of choice.
Note: We recommend that customers contact our technical department for the latest information on the blots as G-Biosciences reserves the right to change the blot profile due to the availability of GenLysate.
Multi tissue blots permit researchers to visualize the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. For example, JIP3, a scaffold protein of the JNK pathway, had a specific brain location and was confirmed to be a neuronal protein (1); human Cds1-related kinase had a testicular localization and was shown to be a meiotic checkpoint kinase (2); the TATA-box binding protein related factor was present in all human tissues (3); Geminin, found solely in testis, was shown to be localized to proliferating cells (6).
An example of our multi tissue; single species blots is shown below (Figure 1). The mouse multi tissue blot (Cat. # TB38) was probed with antibodies against caveolin and the human multi tissue blot (Cat. # TB37-I) was probed with Cox-2.
Blot lane order:
- Protein marker
- Human Normal Liver Lysate
- Human Normal Brain Lysate
- Human Normal Lung Lysate
- Human Normal Kidney Lysate
- Human Normal Spleen Lysate
- Human Normal Testis Lysate
- Human Normal Ovary Lysate
- Human Normal Heart Lysate
- Human Normal Pancreas Lysate
- Bannwarth, S. et al (2014) Brain. doi: 10.1093/brain/awu138
- Gaut, J.P. et al (2014) Clin. Chem. doi: 10.1373/clinchem.2013.212993
- Hayashi, H. et al (2012) J Biol Chem 2012; 287:15054
- Laterza, O.F. et al (2006) Clin. Chem. 52:1713
- Tan, W. et al (2005) Nuc Acid Res 33:3855
- Tohyama, K. et al (2004) Endocrinology 145:4384
- Eward, K.L. et al (J. Cell Sci. 117:5875
- Suzuki, S. et al (2003) Eur. J. Endocrinol. 148:259
- Persengiev, S.P. et al (2003) PNAS. 100:14887
- Barnard, D. et al (2002) RNA 8:526
- Wohlschlegel, J. A. et al (2002) Am J Pathol 161:267
- Tuynder, M. et al (2002) PNAS 99:14976
- Gelman, I.H. et al (2000) histochem. J. 32:13
- Kelkar, N. et al (2000) Mol Cell Biol 20:1030
- Thome, K.C. et al (2000) J. Biol. Chem. 275:35233
- Boettner, B. et al (2000) PNAS 97:9064
- Brown, A.L. et al (1999) PNAS 96:3745
|Human Normal Multiple Tissue Blot; Liver, Brain, Lung, Kidney, Spleen, Testis, Ovary, Heart, Pancreas||1 Blot||