IBS-HP™ Buffer

IBS-HP™ Buffer

The expression of recombinant proteins is a popular and routinely used technique in protein studies.  The expression of recombinant proteins often has one drawback and that is the recombinant proteins aggregate and form inclusion bodies, especially when expressed at high levels.  The aggregated proteins are difficult to solubilize, due to the nature of aggregates, however we offer a selection of products for dealing with the range of issues involved with solubilizing and recovering active proteins from inclusion bodies.

 
Solubilization of Hydrophobic Proteins from Inclusion Bodies
We offer IBS-HP™ Buffer for the solubilization of inclusion bodies containing highly hydrophobic proteins.  The IBS-HP™ Buffer contains optimized concentration of SDS, a buffering agent and urea. After solubilization of inclusion bodies, free SDS may be quenched or removed by competing with a non-ionic detergent.
 
COMPATIBILITY
The IBS-HP™ Buffer is compatible with NI™ Protein Assay (Cat. # 786-005) and, when used with PAGE-Perfect™ kit (Cat. # 786-123) for SDS-PAGE analysis.

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Protocol
786-183HP
Material Safety Data Sheet
786-183HP
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Technical Literature
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Plant Proteomics Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Sample Preparation Handbook For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
Catalog Description Size Price(USD) Qty
786-183HP
IBS-HP™ Buffer 100ml
$174.00