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ITM0152

ITM0152
  • Gene/Protein: CHIT1
  • Product Description: Immunotag™ Chitotriosidase Monoclonal Antibody
368.0000
Price in reward points: 368

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Immunotag™ Chitotriosidase Monoclonal Antibody
Antibody Specification
Datasheet
Target Protein Chitotriosidase
Clonality Monoclonal
Storage/Stability -20°C/1 year
Application WB,ELISA
Recommended Dilution Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Concentration 1 mg/ml
Reactive Species Human
Host Species Mouse
Immunogen Purified recombinant fragment of Chitotriosidase (aa22-137) expressed in E. Coli.
Specificity Chitotriosidase Monoclonal Antibody detects endogenous levels of Chitotriosidase protein.
Purification Affinity purification
Form Ascitic fluid containing 0.03% sodium azide.
Gene Name CHIT1
Accession No. Q13231 Q9D7Q1
Alternate Names CHIT1; Chitotriosidase-1; Chitinase-1
Description chitinase 1(CHIT1) Homo sapiens Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012],
Cell Pathway/ Category Amino sugar and nucleotide sugar metabolism,
Protein Expression Macrophage,Spleen,
Subcellular Localization extracellular region,extracellular space,lysosome,
Protein Function catalytic activity:Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.,disease:Very high plasma levels of CHIT1 are found in patients with Gaucher disease type 1 (GD I). This can be used as diagnostic aid and to evaluate the success of treatment. Successful therapy brings the CHIT1 activity levels back to normal.,function:Degrades chitin and chitotriose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.,polymorphism:A 24 bp duplication in exon 10 leads to the activation of an alternative splice site and the production of an inactive protein. About 6% of the population are deficient for CHIT1 activity, while 35% are carriers and show reduced enzyme levels. People with CHIT1 deficiency appear perfectly healthy.,similarity:Belongs to the glycosyl hydrolase 18 family.,similarity:Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.,similarity:Contains 1 chitin-binding type-2 domain.,subcellular location:A small proportion is lysosomal.,subunit:Monomer.,tissue specificity:Detected in spleen. Secreted by cultured macrophages.,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
Material Safety Data Sheet
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