ITM0480
ITM0480
- Catalog: ITM0480
- Gene/Protein: NOS3
- Product Description: Immunotag™ NOS3 Monoclonal Antibody
504.0000
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Antibody Specification | |
Datasheet | |
Target Protein | NOS3 |
Clonality | Monoclonal |
Storage/Stability | -20°C/1 year |
Application | IHC-p,ELISA |
Recommended Dilution | Immunohistochemistry: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human |
Host Species | Mouse |
Immunogen | Purified recombinant fragment of human NOS3 expressed in E. Coli. |
Specificity | NOS3 Monoclonal Antibody detects endogenous levels of NOS3 protein. |
Purification | Affinity purification |
Form | Ascitic fluid containing 0.03% sodium azide. |
Gene Name | NOS3 |
Accession No. | P29474 P70313 |
Alternate Names | NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
Description | nitric oxide synthase 3(NOS3) Homo sapiens Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009], |
Cell Pathway/ Category | Regulates Angiogenesis, AMPK, PI3K/Akt, Protein_Acetylation |
Protein Expression | Artery,Placenta,Platelet,Umbilical vein, |
Subcellular Localization | Golgi membrane,nucleus,cytoplasm,cytosol,cytoskeleton,plasma membrane,caveola,endocytic vesicle membrane, |
Protein Function | catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like domain.,subcellular location:Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.,subunit:Homodimer. Interacts with NOSIP and NOSTRIN.,tissue specificity:Platelets, placenta, liver and kidney., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |