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ITM1251

  • Gene/Protein: akt
  • Product Description: Immunotag™ Akt(pan) mouse mAb
  • 294.0000
    Price in reward points: 294

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ITM1251

Immunotag™ Akt(pan) mouse mAb
Antibody Specification
Datasheet
Target Protein AKT (Pan)
Clonality Monoclonal
Storage/Stability -20°C/1 year
Application WB
Recommended Dilution wb 1:1000
Concentration 1 mg/ml
Reactive Species Mouse,Rat
Host Species Mouse
Immunogen Purified recombinant human AKT1 protein fragments expressed in E.coli.
Specificity This antibody detects endogenous levels of total AKT and does not cross-react with related proteins.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene Name akt
Accession No. P31749;P31751;Q9Y243
Alternate Names AKT;AKT1;AKT1 kinase;AKT2;AKT2 kinase;Akt3;AKT3_HUMAN;CAKT;DKFZp434N0250;kinase Akt1; MGC99656;Murine thymoma viral (v-akt) homolog 2;PKB;PKB gamma;PKB-GAMMA;PKB/Akt; PKBALPHA;PKBB;PKBBETA;PKBG;PKBGAMMA;PRKBA;PRKBB;PRKBG;Protein kinase Akt-3;Protein kinase B;Protein kinase B gamma;RAC;rac protein kinase alpha;rac protein kinase beta; RAC-gamma;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;RACALPHA;RACalpha serine/threonine kinase;RACBETA;RACgamma;RACgamma serine/threonine protein kinase; RACPKgamma;serine threonine protein kinase;STK-2;STK2;thymoma viral proto oncogene 1; thymoma viral proto oncogene;vakt murine thymoma viral oncogene homolog 1;vakt murine thymoma viral oncogene homolog 2;vakt murine thymoma viral oncogene homolog 3.
Description catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in AKT1 are associated with breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime.,disease:Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].,disease:Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1).,domain:Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.,domain:The AGC-kinase C-terminal mediates interaction with THEM4.,enzyme regulation:Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.,function:General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.,PTM:Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A.,subunit:Interacts with AGAP2 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN and THEM4. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression.,tissue specificity:In all human cell types so far analyzed.,
Cell Pathway/ Category Akt_PKB,MAPK_ERK_Growth,MAPK_G_Protein,ErbB_HER,Chemokine,mTOR,Apoptosis_Inhibition,Apoptosis_Mitochondrial,Apoptosis_Overview,VEGF,Focal adhesion,Tight junction,Toll_Like,Jak_STAT,T_Cell_Receptor,B_Cell_Antigen,Fc epsilon RI,Fc gamma R-mediated phagocytosis,Neurotrophin,Insulin_Receptor,Progesterone-mediated oocyte maturation,Adipocytokine,Pathways in cancer,Colorectal cancer,Renal cell carcinoma,Pancreatic cancer,Endometrial cancer,Glioma,Prostate cancer,Melanoma,Chronic myeloid leukemia,Acute myeloid leukemia,Small cell lung cancer,Non-small cell lung cancer,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
Material Safety Data Sheet
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