ITM3520
ITM3520
- Catalog: ITM3520
- Gene/Protein: PRKAA1
- Product Description: Immunotag™ AMPK α1 Monoclonal Antibody(5G11)
405.0000
Price in reward points: 405
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Antibody Specification | |
Datasheet | |
Target Protein | AMPK α1 (5G11) |
Clonality | Monoclonal |
Storage/Stability | -20°C/1 year |
Application | WB,IHC-p |
Recommended Dilution | WB 1:1000-2000, IHC 1:50-100 |
Concentration | 1 mg/ml |
Reactive Species | Human,Rat,Mouse |
Host Species | Mouse |
Immunogen | Synthetic Peptide of AMPK α1 |
Specificity | AMPK α1 protein detects endogenous levels of AMPK α1 |
Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | PRKAA1 |
Accession No. | Q13131 Q5EG47 P54645 |
Alternate Names | PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1 |
Description | protein kinase AMP-activated catalytic subunit alpha 1(PRKAA1) Homo sapiens The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008], |
Cell Pathway/ Category | Regulation of autophagy,mTOR,Insulin_Receptor,Adipocytokine,Hypertrophic cardiomyopathy (HCM), |
Protein Expression | Brain,Intestine,Liver,Mammary gland,Platelet,Testis |
Subcellular Localization | intracellular,nucleus,nucleoplasm,cytoplasm,cytosol,apical plasma membrane,nucleotide-activated protein kinase complex, |
Protein Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.,function:Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |