Datasheet |
|
Target Protein |
CofIL-in (Ser3) |
Clonality |
Polyclonal |
Storage/Stability |
-20°C/1 year |
Application |
WB,IHC-p,ELISA |
Recommended Dilution |
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
Concentration |
1 mg/ml |
Reactive Species |
Human,Mouse,Rat |
Host Species |
Rabbit |
Immunogen |
Synthesized phospho-peptide around the phosphorylation site of human Cofilin (phospho Ser3) |
Specificity |
Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3. |
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name |
CFL1 |
Accession No. |
P23528 P18760 P45592 |
Alternate Names |
CFL1; CFL; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform |
Description |
cofilin 1(CFL1) Homo sapiens The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004], |
Cell Pathway/ Category |
Axon guidance,Fc gamma R-mediated phagocytosis,Regulates Actin and Cytoskeleton, |
Protein Expression |
B-cell lymphoma,Brain,Cajal-Retzius cell,Epithelium,Fetal brain cortex,Lung,Ovary,Placenta,Platelet |
Subcellular Localization |
extracellular space,nucleus,cytoplasm,focal adhesion,actin cytoskeleton,membrane,nuclear matrix,extracellular matrix,lamellipodium membrane,vesicle,ruffle membrane,extracellular exosome, |
Protein Function |
function:Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.,online information:Cofilin entry,PTM:Phosphorylated on Ser-3 in resting cells.,similarity:Belongs to the actin-binding proteins ADF family.,similarity:Contains 1 ADF-H domain.,subcellular location:Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.,tissue specificity:Widely distributed in various tissues., |
Usage |
For Research Use Only! Not for diagnostic or therapeutic procedures. |