ITP0453
ITP0453
- Catalog: ITP0453
- Gene/Protein: PXN
- Product Description: Immunotag™ Paxillin (phospho Ser178) Polyclonal Antibody
490.0000
Price in reward points: 490
| Antibody Specification | |
| Datasheet | 
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| Target Protein | PaxIL-lin (Ser178) |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Paxillin (phospho Ser178) |
| Specificity | Phospho-Paxillin (S178) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at S178. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | PXN |
| Accession No. | P49023 Q8VI36 Q66H76 |
| Alternate Names | PXN; Paxillin |
| Description | paxillin(PXN) Homo sapiens This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011], |
| Cell Pathway/ Category | Chemokine,VEGF,Focal adhesion,Leukocyte transendothelial migration,Regulates Actin and Cytoskeleton, |
| Protein Expression | Brain,Epithelium,Lung,Placenta,T-cell,Uterus, |
| Subcellular Localization | stress fiber,nucleoplasm,cytoplasm,cytosol,cytoskeleton,microtubule associated complex,plasma membrane,focal adhesion,cell cortex,lamellipodium, |
| Protein Function | function:Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).,PTM:Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.,similarity:Belongs to the paxillin family.,similarity:Contains 3 LIM zinc-binding domains.,similarity:Contains 4 LIM zinc-binding domains.,subunit:Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 (By similarity). Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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