ITP0910
ITP0910
- Catalog: ITP0910
- Gene/Protein: BAD
- Product Description: Immunotag™ Bad (phospho Ser134) Polyclonal Antibody
490.0000
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Antibody Specification | |
Datasheet | |
Target Protein | BAD (Ser134) |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | WB,IHC-p,IF,ELISA |
Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Rat |
Host Species | Rabbit |
Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Bad (phospho Ser134) |
Specificity | Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | BAD |
Accession No. | Q92934 Q61337 O35147 |
Alternate Names | BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter |
Description | BCL2 associated agonist of cell death(BAD) Homo sapiens The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008], |
Cell Pathway/ Category | ErbB_HER,Apoptosis_Inhibition,Apoptosis_Mitochondrial,Apoptosis_Overview,VEGF,Focal adhesion,Neurotrophin,Insulin_Receptor,Alzheimer's disease,Amyotrophic lateral sclerosis (ALS),Pathways in cancer,Colorectal cancer,Pancreatic cancer,Endometrial cancer,Prostate cancer,Melanoma,Chronic myeloid leukemia,Acute myeloid leukemia,Non-small cell lung cancer, |
Protein Expression | Bone marrow,Lung,Skeletal muscle,White Matter pool- 5 brain tissues |
Subcellular Localization | mitochondrion,mitochondrial outer membrane,cytosol, |
Protein Function | domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.,online information:Bcl 2-associated death promoter entry,PTM:Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.,similarity:Belongs to the Bcl-2 family.,subcellular location:Upon phosphorylation, locates to the cytoplasm.,subunit:Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins.,tissue specificity:Expressed in a wide variety of tissues., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |