ITT0101
ITT0101
- Catalog: ITT0101
- Gene/Protein: ACTN1/2/3/4
- Product Description: Immunotag™ Actinin-α1/2/3/4 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | Actinin-α1/2/3/4 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from Actinin-α1/2/3/4, at AA range: 40-120 |
| Specificity | Actinin-α1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-α1/2/3/4 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | ACTN1/2/3/4 |
| Accession No. | P12814/P35609/Q08043/O43707 Q9Z1P2/Q9QXQ0 |
| Alternate Names | ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-linking protein; ACTN3; Alpha-actinin-3; A |
| Description | actinin alpha 1(ACTN1) Homo sapiens Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Focal adhesion,Adherens_Junction,Adherens_Junction,Leukocyte transendothelial migration,Regulates Actin and Cytoskeleton,Systemic lupus erythematosus,Arrhythmogenic right ventricular cardiomyopathy (ARVC), |
| Protein Expression | Colon,Epithelium,Hepatocyte,Neuroblastoma,Placenta,Platelet,Skin,Tumor of tongue, |
| Subcellular Localization | stress fiber,ruffle,extracellular region,extracellular space,intracellular,cytoplasm,cytosol,actin filament,plasma membrane,brush border,cell-cell junction,fascia adherens,focal adhesion,Z disc,platelet |
| Protein Function | function:F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.,similarity:Belongs to the alpha-actinin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 spectrin repeats.,subcellular location:Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.,subunit:Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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