ITT0125
ITT0125
- Catalog: ITT0125
- Gene/Protein: ADD1/ADD2
- Product Description: Immunotag™ Adducin α/β Polyclonal Antibody
385.0000
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Antibody Specification | |
Datasheet | |
Target Protein | Adducin α/β |
Clonality | Polyclonal |
Storage/Stability | -20°C/1 year |
Application | WB,IHC-p,IP,IF,ELISA |
Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
Concentration | 1 mg/ml |
Reactive Species | Human,Mouse,Rat |
Host Species | Rabbit |
Immunogen | Synthesized peptide derived from Adducin α/β, at AA range: 670-750 |
Specificity | Adducin α/β Polyclonal Antibody detects endogenous levels of Adducin α/β protein. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Gene Name | ADD1/ADD2 |
Accession No. | P35611/P35612 Q63028/Q05764 |
Alternate Names | ADD1; ADDA; Alpha-adducin; Erythrocyte adducin subunit alpha; ADD2; ADDB; Beta-adducin; Erythrocyte adducin subunit beta |
Description | adducin 1(ADD1) Homo sapiens Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq, Jul 2008], |
Protein Expression | Brain cortex,Epithelium,Muscle,Platelet,Reticulocyte,Testis, |
Subcellular Localization | nucleus,nucleoplasm,cytosol,cytoskeleton,plasma membrane,cell-cell adherens junction,focal adhesion,F-actin capping protein complex, |
Protein Function | Additional isoforms seem to exist,domain:Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.,function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.,PTM:The N-terminus is blocked.,similarity:Belongs to the aldolase class II family. Adducin subfamily.,subunit:Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit. Binds ROCK1.,tissue specificity:Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit., |
Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |