ITT2960
ITT2960
- Catalog: ITT2960
- Gene/Protein: NRAS/HRAS/KRAS
- Product Description: Immunotag™ N/H/K-Ras Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | N/H/K-Ras |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from N/H/K-Ras, at AA range: 10-90 |
| Specificity | N/H/K-Ras Polyclonal Antibody detects endogenous levels of N/H/K-Ras protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | NRAS/HRAS/KRAS |
| Accession No. | P01111/P01112/P01116 Q04970/P20171/P08644 |
| Alternate Names | NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras |
| Description | neuroblastoma RAS viral oncogene homolog(NRAS) Homo sapiens This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011], |
| Cell Pathway/ Category | MAPK_ERK_Growth,MAPK_G_Protein,ErbB_HER,Chemokine,Axon guidance,VEGF,Tight junction,Gap junction,Natural killer cell mediated cytotoxicity,T_Cell_Receptor,B_Cell_Antigen,Fc epsilon RI,Long-term potentiation,Neurotrophin,Long-term depression,Regulates Actin and Cytoskeleton,Insulin_Receptor,GnRH,Melanogenesis,Pathways in cancer,Renal cell carcinoma,Endometrial cancer,Glioma,Prostate cancer,Thyroid cancer,Melanoma,Bladder cancer,Chronic myeloid leukemia,Acute myeloid leukemia,Non-small cell lung cancer, |
| Protein Expression | Bone marrow,Bone-marrow,Brain,Fibrosarcoma,Kidney,Leukemia,Lung car |
| Subcellular Localization | Golgi membrane,intracellular,Golgi apparatus,plasma membrane,membrane,extracellular exosome, |
| Protein Function | disease:Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.,disease:Mutations which change AA 12, 13 or 61 activate the potential of Ras to transform cultured cells and are implicated in a variety of human tumors.,enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).,function:Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.,online information:NRAS mutation db,online information:RAS proteins entry,PTM:Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.,similarity:Belongs to the small GTPase superfamily. Ras family.,subcellular location:Shuttles between the plasma membrane and the Golgi apparatus., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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