ITT3906
ITT3906
- Catalog: ITT3906
- Gene/Protein: SPI1
- Product Description: Immunotag™ PU.1 Polyclonal Antibody
385.0000
Price in reward points: 385
| Antibody Specification | |
| Datasheet | 
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| Target Protein | PU.1 |
| Clonality | Polyclonal |
| Storage/Stability | -20°C/1 year |
| Application | WB,IHC-p,IF,ELISA |
| Recommended Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Concentration | 1 mg/ml |
| Reactive Species | Human,Mouse,Rat,Monkey |
| Host Species | Rabbit |
| Immunogen | Synthesized peptide derived from PU1, at AA range: 150-230 |
| Specificity | PU.1 Polyclonal Antibody detects endogenous levels of PU.1 protein. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Gene Name | SPI1 |
| Accession No. | P17947 P17433 Q6BDS1 |
| Alternate Names | SPI1; Transcription factor PU.1; 31 kDa-transforming protein |
| Description | Spi-1 proto-oncogene(SPI1) Homo sapiens This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Cell Pathway/ Category | Pathways in cancer,Acute myeloid leukemia, |
| Subcellular Localization | nuclear chromatin,integral component of membrane, |
| Protein Function | function:Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.,induction:Highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the Spi-1 gene due to the insertion of SFFV.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds DNA as a monomer. Interacts with RUNX1 and SPIB. Interacts with CEBPD and NONO., |
| Usage | For Research Use Only! Not for diagnostic or therapeutic procedures. |
| Material Safety Data Sheet | |
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