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ITT3964

ITT3964
  • Gene/Protein: RAD51
  • Product Description: Immunotag™ Rad51 Polyclonal Antibody
280.0000
Price in reward points: 294

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Immunotag™ Rad51 Polyclonal Antibody
Antibody Specification
Datasheet
Target Protein Rad51
Clonality Polyclonal
Storage/Stability -20°C/1 year
Application WB,IHC-p,ELISA
Recommended Dilution Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Concentration 1 mg/ml
Reactive Species Human,Mouse
Host Species Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human RAD51. AA range:275-324
Specificity Rad51 Polyclonal Antibody detects endogenous levels of Rad51 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene Name RAD51
Accession No. Q06609 Q08297
Alternate Names RAD51; RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog A
Description RAD51 recombinase(RAD51) Homo sapiens The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009],
Cell Pathway/ Category Homologous recombination,Pathways in cancer,Pancreatic cancer,
Protein Expression Placenta,Testis,
Subcellular Localization nuclear chromosome,nuclear chromosome, telomeric region,chromatin,nuclear chromatin,condensed chromosome,condensed nuclear chromosome,lateral element,nucleus,nucleoplasm,nucleolus,cytoplasm,mitochondrion,mitochondrial
Protein Function disease:Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].,function:May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.,PTM:Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.,similarity:Belongs to the recA family.,similarity:Belongs to the recA family. RAD51 subfamily.,similarity:Contains 1 HhH domain.,subcellular location:Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.,subunit:Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer (By similarity). Interacts with OBFC2B.,tissue specificity:Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.,
Usage For Research Use Only! Not for diagnostic or therapeutic procedures.
Material Safety Data Sheet
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