FOCUS™ Glycoprotein (2 Citations)
FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A) bound resin with the capacity to bind and immobilize ~20mg glycoproteins. Column bound glycoproteins are eluted with a set of three rapid elution buffers. The proprietary serial elution allows for faster elution compared to other glycoprotein purification systems.
The eluted proteins are suitable for 2D electrophoresis and isoelectric focusing.
Spin column protocol
Uses a high capacity lectin binding resin (10-16mg/ml resin)
Elution of glycoproteins within 90 minutes with a set of three rapid elution buffers
Fractionation and enrichment of glycoproteins suitable for 2D eletrophoresis and other applications
|Material Safety Data Sheet|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Protein Purification Handbook|
|Rapid Fractionation & Enrichment of Glycoproteins for Analysis by 2D Electrophoresis|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|