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Recombinant Protein A/G & Immobilized Protein A/G (5 Citations)

Recombinant Protein A/G contains four Fc-binding domains from Protein A and two from Protein G but lacks the albumin binding sites. 

Immobilized Protein A/G consists of recombinant protein A/G ligand covalently immobilized onto 6% highly cross-linked agarose.  The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.

Protein A/G binds well to IgG subclasses but does not bind IgA, IgM or serum albumin. This makes Protein A/G an excellent tool for purification and detection of monoclonal antibodies from IgG subclasses, without interference from IgA, IgM and serum albumin. Individual subclasses of monoclonals are likely to have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G.
 
The Immobilized Protein A/G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample.
 
The Immobilized Protein A/G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample.
 
Specific binding and elution buffers are also available.

 

Click here to request bulk or custom sizes.

 


Features

  • High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin
  • Ligand: Recombinant Streptococcal protein A/G lacking the albumin binding sites expressed in E. coli
  • Bead size: 50-165μm
  • Bead Structure: 6% highly cross-linked agarose

 


Applications

  •  Suitable for immunoaffinity chromatography and immunoprecipitation.

 

 

Protocol
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Material Safety Data Sheet
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786-837
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786-839
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786-840
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786-841
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786-2012
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Technical Literature
Protein A, Protein G, Protein A/G and Protein L Binding Affinity A table highlighting the binding affinities of protein A, protein G, protein A/G and Protein L.  Designed to help in the choice of Well-Coated™ plates.

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