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Immobilized Protein G

Catalog Description Size Price(USD) Qty
786-829
Immobilized Protein G Resin 2ml resin
$220.00
786-284
Immobilized Protein G Resin 5ml Resin
$480.00
786-830
Immobilized Protein G Resin 10ml resin
$890.00
786-831
Immobilized Protein G Resin 25ml resin
$1,801.00
786-832
Immobilized Protein G Resin 5 x 1ml Columns
$536.00
786-833
Immobilized Protein G Resin 5 column kit
$714.00
786-834
Immobilized Protein G Resin 10 x 0.2ml columns
$304.00
786-835
Immobilized Protein G Resin 10 column kit
$392.00
786-997
Protein G Resin Spin Plate 1 Plate
$240.00

Immobilized Protein G

Immobilized protein G is for binding the constant domains of immunoglobulin (Ig) molecules (see Figure 1). It is ideal for the easy one-step purification of classes, subclasses and fragments of immunoglobulins for biological fluids and cell culture media. Protein G is a modified form of Streptococcal group G so that it does not bind to albumin. Protein G is coupled to 4% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for Ig and minimal protein G leaching. Our immobilized protein G has a high binding capacity (>20mg sheep IgG/ml resin).

 

Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

 

The Immobilized Protein G 0.2ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 0.2ml column enables purification of 0.1-1mg IgG from 25-500µl of serum or other sample.

 

The Immobilized Protein G 1ml spin column kit is ideal for the small scale affinity purification of antibodies form a variety of samples.  Each 1ml column enables purification of 1-13mg IgG from 0.5-2ml of serum or other sample.

 

Also available in 96-well spin plate formats for processing up to 96 samples.

 

Specific binding and elution buffers are also available.

 

Click here to request bulk or custom sizes.

 

 


Features

  • High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin
  • Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding domain produced in E. coli
  • Bead size: 50-165μm
  • Bead Structure: 4% highly cross-linked agarose
  • Maximum flow rate 1,000cm/hr
  • Maximum pressure 300kPa

 


Applications

  • Suitable for immunoaffinity chromatography and immunoprecipitation.

 

 

Protocol
786-829
786-284
786-830
786-831
786-832
786-833
786-834
786-835
786-997
Material Safety Data Sheet
786-829
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-284
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-830
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-831
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-832
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-833
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-834
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-835
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-997
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Antibody Handbook
Clean, Simple, Rapid Purification of Antibodies
Mass Spectrometry Sample Prep Handbook A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.
Protein A, Protein G, Protein A/G and Protein L Binding Affinity A table highlighting the binding affinities of protein A, protein G, protein A/G and Protein L.  Designed to help in the choice of Well-Coated™ plates.
Protein Electrophoresis Handbook A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.
Protein Purification Handbook
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