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Immobilized DNase I

Immobilized_DNase_I

Immobilized DNase I is designed for use in molecular biology to remove DNA from RNA samples for RT-PCR purposes. Some RT-PCR reactions require removal of DNA from samples to prevent their interference in RT-PCR reactions. Samples can be directly treated with DNase I, however this adds addition step of removing DNase I. Immobilized DNase I thus is preferred to remove DNA from samples.

 

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Features:

  • Matrix: 6% cross-linked Agarose
  • ​Coupling is covalent through linkage of amino group of the DNase I to the pre-activated agarose.
  • Extent of labeling: 0.8-1.2 mg DNase I per ml resin
  • Storage buffer: 50% Glycerol PBS
  • Storage Temperature: -20°C, avoid repeated thaw cycles, store in aliquots
  • Available in 2 ml resin size

Application:

  • Immobilized DNase I agarose is designed to remove DNA from the samples without contaminating samples with DNase I

 

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