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Immobilized Methotrexate

Immobilized_Methotrexate

Immobilized Methotrexate is designed for affinity purification of enzyme dihydrofolate reductase (DHFR). DHFR has been purified from a variety of sources by conventional protein purification procedures. However due to small amount of DHFR activity present in cells and tissues, the conventional methods result in low yield. Methotrexate acts as a potent inhibitor of DHFR. Therefore, the Immobilized Methotrexate binding of DHFR results in higher yields and high purity. 

 

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Features:

  • ​Matrix: 6% cross-linked agarose
  • Matrix acivation: epoxy
  • Extent of labeling: 2-7 mg/ml resin
  • Storage buffer: 20% ethanol  

Application(s):

  • Immobilized Methotrexate is used for affinity purification of enzyme dihydrofolate reductase (DHFR).
Material Safety Data Sheet
786-1815
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786-1816
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish

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