Immobilized Triton X-100
Catalog
Description
Size
Price(USD)
Qty
Catalog
786-1826
786-1826
Description
Immobilized Triton X-100
Immobilized Triton X-100
Size
10 ml resin
10 ml resin
$457.00
$457.00
Catalog
786-1827
786-1827
Description
Immobilized Triton X-100
Immobilized Triton X-100
Size
100 ml resin
100 ml resin
$3,656.00
$3,656.00
Immobilized Triton X-100 is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism. The misfolded proteins or inclusion bodies are solubilized with denaturants such as guanidine hydrochloride, 6M. The solubilized protein is captured by Triton X-100 when passed through immobilized Triton X-100 column. The elution of protein is carried out by increasing β-cyclodextrin concentration. β-cyclodextrin allows for proper refolding of the protein.
Features:
- Active group: Triton X-100
- Extent of labeling: 15-30 µmol /g of drained gel
- Matrix: 6% cross-linked agarose
- pH stability: 6-9
- Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Do not use azides.
- Storage temperature: 4°C
- Supplied as 50% slurry in 20 % ethanol
Application(s):
- Immobilized Triton X-100 is use for refolding of recombinant proteins that were expressed in aggregated form or as inclusion bodies in genetically engineered cells such as E. coli.