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Western Blot to Identify Alpha-Amylase (Lab 6G)

This kit is designed to teach 8 groups of four students the principles of Western blotting.

 

The kit contains the reagents to allow students to:

  • Conduct a serial dilution of a stock amylase solution
  • Prepare samples for PAGE electrophoresis
  • Separate proteins by electrophoresis
  • Transfer proteins to a membrane via Western transfer (Western Blot)
  • Detect and visualize protein of interest (amylase) using enzyme-labeled antibody


Features

  • For 8 groups of 4 students 
  • Requires PAGE electrophoresis equipment with Western blotting module, micropipettes and tips


Applications

  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

NOTE: This kit is supplied with premade gels that are compatible with most other 10cm tanks.  For Mini Protean™ and XCell SureLock™ gel boxes please contact us to request the appropriate substitution.

 

Protocol
BTNM-6E
Material Safety Data Sheet
BTNM-6E
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