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Well-Coated™ Streptavidin

Catalog Description Size Price(USD) Qty
786-745
Well-Coated™ Streptavidin, 8-well strip plate, Clear 5 Plates
$203.00
786-778
Well-Coated™ Streptavidin, 96 well plate, Black 5 Plates
$205.00
786-779
Well-Coated™ Streptavidin, 96 well plate, White 5 Plates
$205.00

Well-Coated™ Streptavidin

Well-Coated™ Streptavidin plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies. This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. 

 

Biotin exhibits an extraordinary binding affinity for avidin (Ka=1015M-1) and streptavidin (Ka=1015 M-1). Biotin and avidin interaction is rapid and once the bond is established it can survive up to 3M guanidine-hydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH1.5 or by autoclaving. Streptavidin is in many respects is similar to avidin except that it has no carbohydrate and the solubility of streptavidin (isoelectric pH5) in aqueous buffer is much lower than avidin. The binding of streptavidin is similar to that of avidin with less non-specific binding due to the lack of carbohydrate groups. 

 

Well-Coated™ Streptavidin plates are suitable for direct, indirect, competitive and sandwich assays. The wells are coated to a 100µl depth and are supplied pre-blocked in our proprietary Superior™ Blocking Buffer. The clear, white and black plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively.
 

 


Features

  • High binding capacity for biotin
  • Low non-specific binding
  • Ideal for peptides, antibodies and small hydrophilic molecules
  • Reduced non-specific binding as plates are pre-blocked with Superior™ Blocking Buffer

 

 

 

Protocol
786-745
786-778
786-779
Material Safety Data Sheet
786-745
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English_UK
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786-778
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
786-779
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
Swedish
Polish
Technical Literature
Assay Development A handbook for selection of products for assay development, including coated plates, blocking agents, antibodies, enzymes and antibody labeling tools.
Bioassays Handbook
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