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HOOK™ Activated Agarose (Amine Reactive)

Catalog Description Size Price(USD) Qty
786-066
HOOK™ Activated Agarose (Amine Reactive) 10ml Resin
$146.00

HOOK™ Activated Agarose (Amine Reactive)

For the generation of peptide and protein affinity columns, for the purification of antibodies and for the discovery of important interacting proteins and cofactors. G-Biosciences offers activated agarose for the coupling of peptides and proteins to agarose through their primary amines.
 
The amine reactive HOOK Activated Agarose is 6% agarose that has been activated to generate reactive aldehyde groups. The aldehyde groups of the agarose react spontaneously with primary amines, located at the N-terminus of proteins or in lysine residues, to form intermediate Schiff Base complexes. These, in turn, are selectively reduced by reductive amination, using sodium cyanoborohydride to form stable amine linkages between the agarose and the ligand.
 

 


Features

  • Binding capacity: 20mg protein/ml resin
  • 6% cross-linked agarose

 



Applications

  • Coupling of proteins and peptides to agarose beads 
  • Suitable for antibody purification.
  • Discovery of new interacting proteins.

 

 

Protocol
786-066
Material Safety Data Sheet
786-066
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Technical Literature
Antibody Handbook
Generation of Protein Ligand Affinity Columns That Reduce Protein Leaching.
Plant Proteomics Handbook
Protein Labeling & Conjugation
Protein Purification Handbook
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