Protein labeling is an important technique that allows researchers to study a protein’s location, movement and interactions within a living cell. The most common labels used are fluorescent labels that allow researchers to study proteins with fluorescent microscopy techniques. Fluorescent labels are used to label proteins directly or, more commonly, to label antibodies or antigens and then follow the antigen:antibody complex in living cells.
This kit allows students to label a protein with green (FITC) and red (Rhodamine) fluorescent labels, which allows students to understand the principle of colocalization, the production of a different color when two different fluorescent labels localize to the same area. This kit uses protein electrophoresis and a UV lamp or light box to visualize the result, so no expensive fluorescent microscopes are required.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
- Fluorescence labeling of antibody:antigen complex.
- Label proteins with fluorescent red and green labels.
- Understand importance of protein labeling.
- Teaches principle of colocalization.