Saline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer.
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- 0.3M trisodium citrate (adjusted to pH 7.0 with HCl)
- 3M sodium chloride