Saline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. For more hybridization buffers, click here.
A dry format 2X SSC Buffer is available to prepare 20 x 1L of 300mM NaCl, 30mM Sodium citrate,pH 7.0
- 0.3M trisodium citrate (adjusted to pH 7.0 with HCl)
- 3M sodium chloride