Agarose II (Low Melting)

Agarose II (Low Melting)
Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice. 
 
Specifications
Moisture
≤ 7%
Ash
≤ 0.4%
EEO (electroendosmosis)
≤ 0.12
Sulfate
≤ 0.12%
Clarity 1.5% (NTU)
≤ 4
Gel Strength 1.5% (g/cm2)
≥ 500
Gelling Temperature 1.5% (ºC)
24-28
Melting Temperature 1.5% (ºC)
≤ 65.5
DNase/ RNase Activity
None Detected
DNA Resolution ≥1000bp
Finely resolved
Gel background
Very low
 
Appropriate Agarose Concentrations
GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER
0.75 500-20,000bp 500-12,000bp
1.00 300-16,000bp 300-8,000bp
1.25 250-10,000bp 200-4,000bp
1.50 200-5,000bp 150-3,000bp
1.75 100-2,500bp 100-2,000bp
2.0 50-1,500bp 50-1,000bp


Features

  • Lower gel strength compared to standard agarose
  • Higher clarity
  • Easy to handle


Applications

  • Electrophoresis of DNA Fragments >1000bp
  • Tissue and Cell Culture
  • Viral Plaque Assays

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SDS
RC-007
RC-008
Other Technical Literatures Description
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Catalog Description Size Price(USD) Qty
RC-007
Agarose II (Low Melting agarose) 25g
$142.00
RC-008
Agarose II (Low Melting agarose) 100g
$333.00