XIT™ Genomic DNA from Buccal Cells (3 Citations)
The XIT™ Genomic DNA from Buccal Cells kit is designed for the isolation of genomic DNA from Buccal cells (cheek cells).
The kit uses three main steps (see figure) that are rapid & efficient cell lysis, enzymatic protein digestion and complete protein precipitation, followed by DNA precipitation. This yields high quality genomic DNA.
The kit is available with (Cat. # 786-343, 786-344) or without (Cat. # 786-341, 786-342) cheek cell collection brushes. The kits without the brushes uses a mouthwash method to collect Buccal cells.
The kits are available in two different sizes. The purified DNA has a A260/A280 ratio between 1.8 and 2.0 and is up to 200kb in size. The yield is approximately 3-5µg/swab.
Isolate high quality genomic DNA
Protocols for brush or mouthwash cheek cell collection
Yield is approximately 3-5µg/swab
Suitable for archive quality DNA.
|Material Safety Data Sheet|
|Molecular Biology Handbook||A guide to our products for DNA and RNA.|
- Willis, JR. et al (2020) The Human Oral Microbiome in Health and Disease: From Sequences to Ecosystems. Microorganisms. 8(2):308
- Tyagi, A. et al (2019) Genetic Landscape of Mitochondrial Regulatory Region in Pediatric Acute Myeloid Leukemia: Changes from Diagnosis to Relapse. J Pediatr Genet. 08(04):193-197
- Tyagi, A. et al (2019)Prognostic impact of mitochondrial DNA D-loop variations in pediatric acute myeloid leukemia. Oncotarget. doi: 10.18632/oncotarget.26665