The XIT™ Mitochondrial DNA kit combines our FOCUS™ Mitochondria technology with our XIT™ DNA isolation technology in a single kit.
The kit uses our proprietary Subcell buffers to lyses cells and animal tissue and remove cellular proteins and nuceli from a highly enriched, intact and active mitochondrial fraction. The mitochondria are then lyzed with the included lysis buffer.
The lysate is treated with our LongLife™ Proteinase K to degrade the proteins, which are then precipitated and the mitochondrial DNA is isolated with alcohol precipitation.
The kits is designed for ~100 preps consiting of 20x106 mammalian cells/ prep. The kit protocol also contains protocols for isolation of mitochondra from soft (i.e. brain & liver) and hard (i.e. cardiac & skeletal muscle) tissue.
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