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XIT™ Mitochondrial DNA (1 Citations)

The XIT™ Mitochondrial DNA kit combines our FOCUS™ Mitochondria technology with our XIT™ DNA isolation technology in a single kit.

 

The kit uses our proprietary Subcell buffers to lyses cells and animal tissue and remove cellular proteins and nuceli from a highly enriched, intact and active mitochondrial fraction.  The mitochondria are then lyzed with the included lysis buffer.

 

The lysate is treated with our LongLife™ Proteinase K to degrade the proteins, which are then precipitated and the mitochondrial DNA is isolated with alcohol precipitation.

 

The kits is designed for ~100 preps consiting of 20x106 mammalian cells/ prep.  The kit protocol also contains protocols for isolation of mitochondra from soft (i.e. brain & liver) and hard (i.e. cardiac & skeletal muscle) tissue.

 

 


Features

  • Isolate high quality mitochondrial DNA
  • Suitable for cultured cells, soft and hard animal tissue

 

 

Protocol
786-301
Material Safety Data Sheet
786-301
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Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.

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