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Agarose II (Low Melting) (1 Citations)


Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice. 
 
Specifications
  • Moisture: ≤ 7%
  • Ash: ≤ 0.4%
  • EEO (electroendosmosis): ≤ 0.12
  • Sulfate: ≤ 0.12%
  • Clarity 1.5% (NTU): ≤ 4
  • Gel Strength 1.5% (g/cm2): ≥ 500
  • Gelling Temperature 1.5% (ºC): 24-28
  • Melting Temperature 1.5% (ºC): ≤ 65.5
  • DNase/ RNase Activity: None Detected
  • DNA Resolution ≥1000bp: Finely resolved
  • Gel background: Very low
 
Appropriate Agarose Concentrations
GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER
0.75 500-20,000bp 500-12,000bp
1.00 300-16,000bp 300-8,000bp
1.25 250-10,000bp 200-4,000bp
1.50 200-5,000bp 150-3,000bp
1.75 100-2,500bp 100-2,000bp
2.0 50-1,500bp 50-1,000bp

 


Features

  • Lower gel strength compared to standard agarose
  • Higher clarity
  • Easy to handle

 


Applications

  • Electrophoresis of DNA Fragments >1000bp
  • Tissue and Cell Culture
  • Viral Plaque Assays

 

 

Material Safety Data Sheet
RC-007
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RC-008
English_US
Danish
Dutch
English_UK
French
German
Spanish
Norwegian
Portuguese
Finnish
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Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Certificate Of Analysis
RC-007
090607
101106
101503
102010
110802
111008
120401
120905
130404
140805
RC-008
091211
100106
100701
101106
102408
111008
111711
112305
120401
120905
122006
130205
200211
151108

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