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SOB Medium [Hanahan's Broth]

SOB_Medium_Hanahans_Broth


SOB Medium [Hanahan's Broth] is nutrient rich medium used for cultivation of E. coli. It is suitable for preparation of competent E. coli cells often with addition of 0.4% dextrose (SOC medium). It has also been found suitable for preparation of C. necator H16 competent cells.

 

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Features:

  • Composition: 2% Tryptone, 0.5% yeast extract, 10 mM sodium chloride, 2.5 mM potassium chloride, 10 mM Magnesium chloride and 10 mM Magnesium sulfate, pH6.8 ± 0.2
  • Sterile filtered using 0.2 micron filter units.
  • available in 10 x 10 ml, 1 L and 10x 1 L sizes

 

Application(s)

  • Cultivation of E.coli
  • Suitable for preparation of competent E. coli cells often with addition of 0.4% dextrose (SOC medium)
  • Suitable for preparation of C. necator H16 competent cells.

 

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