First strand cDNA synthesis kit (12 Citations)

First strand cDNA synthesis kit is in a 5x master mix form which contains Reverse Transcriptase (RT), recombinant RNase inhibitor, dNTPs and an optimized buffer with protein stabilizers. G-biosciences RT is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. This RT is an RNA-directed DNA polymerase which lacks 3´→ 5´ exonuclease activity and can produce full-length cDNA from long RNA templates.

Items supplied:

• 5X master mix
• Random Hexamer Primer (50µM)
• Oligo (dT)15 (50µM)

Applications:

• First strand cDNA synthesis
• Gene expression analysis

Benefits:

• Active at temperatures up to 55°C.
• Highly efficient at producing full-length cDNA from low RNA concentration.

Storage Temperature: – 20º C