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Bsu DNA Polymerase, Large fragment

Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. It also lacks the 3’→ 5’ exonuclease activity.

Applications

  • Second strand cDNA synthesis
  • Strand displacement DNA synthesis
  • Random primer labeling
  • dA-tailing

Product Source

E. coli strain expressing Bsu DNA Polymerase, Large fragment gene.

Product Includes

  • Bsu DNA Polymerase, Large fragment
  • 10X Bsu DNA Polymerase, Large fragment reaction buffer

10X Bsu DNA Polymerase, Large fragment

100 mM Tris-HCl
500 mM KCl
100 mM MgCl2
10 mM DTT, pH 7.9 @ 25°C

Storage Buffer

50mM Tris-HCl

50 mM KCl

1 mM DTT

0.1 mM EDTA

50% Glycerol, pH 7.5 @ 25ºC

Storage Temperature: – 20ºC

Heat Inactivation: 65°C for 20 min

Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 37º C.

Quality Control assays

Bsu DNA Polymerase, Large fragment is free from detectable nuclease contamination.

Material Safety Data Sheet
786-1998
English_US
786-1999
English_US
786-2000
English_US

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