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T4 UvsY Protein









T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event. UvsY stimulates the DNA-dependent ATPase activity of UvsX, lowers the critical concentration of UvsX that is required for activity, and promotes strand exchange. UvsY efficiently promotes the UvsX-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.                                     

Product Source

E. coli cells expressing T4 UvsY gene

Product Includes

  • T4 UvsY protein
  • 10X UvsY reaction buffer

10X UvsY reaction buffer composition

200 mM Tris-acetate

1M Potassium acetate

100 mM Magnesium acetate

10 mM DTT

pH 7.8 @ 25ºC

Storage Buffer

50 mM Tris-HCl

50 mM KCl

1 mM DTT

0.1 mM EDTA

50% Glycerol

pH 7.5 @ 25ºC

Storage Temperature: -20ºC

Quality Control assays

UvsY is free from detectable nuclease contamination.

Material Safety Data Sheet
786-1992
English_US
786-1993
English_US
786-1994
English_US

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