A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, E-64, Pepstatin A, 1,10-Phenathroline and PMSF that inhibits yeast and fungal serine, cysteine and metalloproteases. This protease inhibitor cocktail is specifically designed for the inhibition of proteases in yeast and fungal cell lysates.
|
Sample Type & key features
|
Inhibition Specificity
|
1X Composition
|
Concentration & Available Sizes
|
|
For yeast and fungal cell extracts
|
Yeast and fungal serine, cysteine and metalloproteases
|
668µM AEBSF
14µM E-64
1µM Pepstatin A
2mM 1,10-Phenathroline
1mM PMSF
|
100X DMSO solution
1ml, 5ml
|
Features
- Yeast and fungal specific protease inhibitor cocktail
- 100X concentrated, ready-to-use solution
- No proprietary ingredients, fully disclosed formulation of five broad range protease inhibitors, including AEBSF, E-64, Pepstain A, 1,10-Phenathroline and PMSF
Applications
- Inhibition of protease activity in protein preparations of yeast and fungal lysates.
- Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays, and other applications.
ProteaseArrest Family
Our ProteaseArrest™ is a highly efficient, liquid format general protease inhibitor cocktail that offers >95% protease inhibition. The ProteaseArrest™ family of inhibitor cocktails includes other species-specific inhibitor cocktails as well, all of which are enhanced with inhibitors specific for that species. In addition to Bacteria, species-specific cocktails are available for Plant, Bacterial and Mammalian samples. Our Recom ProteaseArrest™ is designed for recombinant protein purification, particularly 6X His tagged proteins. These proteins are purified by IMAC (Immobilized Metal Affinity Chromatography), which is severely inhibited by the routinely used metalloproteases inhibitor EDTA. Recom ProteaseArrest™ contains a specific bacterial metalloproteases that does not interfere with 6X His protein purification. Our FOCUS™ ProteaseArrest™ is designed to protect proteins destined for 2D electrophoresis and mass spectrometry analysis. The TCM ProteaseARREST™ protease inhibitor cocktail is a broad range, 200X concentrated, ready-to-use protease inhibitor cocktail sterile filtered for tissue culture media. TCM ProteaseArrest™ inhibits a wide range of serine, cysteine and other specific proteases including aminopeptidases trypsin-like and acid proteases. Designed for the protection of proteins secreted in to the culture media.
