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Yeast Research Tools

Download the Molecular Biology Handbook for Complete Product Listing

 

G-Biosciences offers a large selection of products specifically designed for yeast research, whether working with DNA or looking at proteins. 

Yeast DNA Related Products

Three kits are offered for the extraction of genomic DNA from yeast. OmniPrep™ for Yeast allows for the purification of highly pure genomic DNA, XIT™ genomic DNA from yeast uses lytic digestion and protein precipitation to release high quality DNA and Yeast Geno-DNA-Template™ is ideal for rapid extraction yeast DNA for use as a template in PCR. 

For rapid single step yeast transformation in under a minute our Fast Yeast Transformation Kit is highly efficient and our EZ Yeast™ Plasmid Prep is designed for the rapid isolation of 2μ plasmids from yeast patches or yeast grown in small liquid cultures. Longlife Zymolyase®, a highly stable and ready-to-use yeast lytic enzyme is also available. 

Yeast Protein Related Products

For the extraction of proteins from yeast , two lysis buffer systems are offered to extract active, soluble proteins, PopLysis™ Yeast Lysis and Extraction and Yeast PE LB™. For the extraction of the total yeast proteome the chaotropic FOCUS™ Yeast Proteome is recommended. To preserve your protein of interest, a yeast specific protease inhibitor cocktail should be used, Yeast/ Fungal ™​ProteaseArrest.  Saccharomyces cerevisiae GenLysate™ is a yeast cell lysate of total proteins, extracted from Saccharomyces cerevisiae and is ready to use for SDS-PAGE and Western blotting purposes. Several kits are offered for the extraction and purification of 6X His or GST tagged recombinant proteins from yeast. 

Gene/Protein:
Tranfer RNAs (tRNAs) are used as carriers or co-precipitants for helping in recovery of nucleic acids when alcohol precipitation method is used. Carrier or co-precipitants are inert substances that enhance recovery of nucleic acid when alcohol is used for precipitation. They are insoluble in ethanol..
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Gene/Protein:
GenLysates™ are a tissue/cell lysate of total proteins, extracted from tissue/cells of interest and is ready to use for SDS-PAGE and Western blotting purposes. The total proteins are being extracted from whole tissue or cells following the method of Laemmli1. The proteins are extracted in a de..
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Gene/Protein:
PopLysis™ Yeast Lysis and Extraction buffer is part of our PopLysis™ buffer systems. PopLysis™ buffers are based on cocktail of membrane solubilizing detergents optimized to rapidly solubilize and pop open membrane structures allowing cellular proteins to spill out into lysis solut..
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Gene/Protein:
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail of AEBSF, E-64, Pepstatin A, 1,10-Phenathroline and PMSF that inhibits yeast and fungal serine, cysteine and metalloproteases. This protease inhibitor cocktail is specifically designed for the inhibition of proteases in yeast..
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