Aminobutyl-NTA was first used by Dr. Hochuli in 1987 to purify recombinant proteins (the “Histag” technique). Since then, the AB=NTA has become an indispensable tool for immobilizing proteins with high specificity on solid surfaces, such as glass or the gold electrode. A solid surface is modified by AB-NTA and bio-functionalized via Ni (II) with a genetically expressed protein bearing a hexahistidine extension at its terminus. The His-tag technique has become increasingly important, particularly in surface plasmon resonance and structural analyses of proteins by x-ray interference. Using the His-tag technique, Dr. Noji was able to directly observe the rotation of F1-ATPase by fluorescence microscope.
Features
- CAS#: 941689-36-7
- Chemical Name: N-(5-Amino-1-carboxypentyl)iminodiacetic acid
- Synonym: Nα,Nα-Bis(carboxymethyl)-L-lysine hydrate
- Appearance: White pale yellowish white powder
- Purity: ≥97.0% (HPLC)
- MW: 262.26, C10H18N2O6
- Storage Condition: ambient temperature
- Shipping Condition: ambient temperature
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