The DNAmark™ 500bp ladder is a ready-to-use preparation that consists of 10 DNA fragments ranging from 500bp to 5.0kb. The 2,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5µl loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.