PBST [10X] (8 Citations)

A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na₂HPO₄, 1.5M NaCl, 20mM KH₂PO₄, 30mM KCl, 0.5% Tween® 20, pH 7.4. A dry format PBST is also avaialble

PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.

By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Features

• 1X Concentration is 8mM Na₂HPO₄, 150mM NaCl, 2mM KH₂PO₄, 3mM KCl, 0.05% Tween® 20, pH 7.4

Applications

• Wash buffer for Western Blotting and ELISA